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Image Search Results
Journal: Human Molecular Genetics
Article Title: Selective disruption of Tcf7l2 in the pancreatic β cell impairs secretory function and lowers β cell mass
doi: 10.1093/hmg/ddu553
Figure Lengend Snippet: Glucose and insulin tolerance in Tcf7l2 fl/fl ::Ins1.Cre + mice. Intraperitoneal glucose tolerance was assessed at 8 ( A ), 12 ( B ), 16 ( C ) and 20 ( D ) weeks of age in Tcf7l2 fl/fl ::Ins1.Cre + (blue) and Tcf7l2 fl/fl ::Ins1.Cre − (black dotted) mice maintained on a normal chow diet (ND), and Tcf7l2 fl/fl ::Ins1.Cre + (red) and Tcf7l2 fl/fl ::Ins1.Cre − (black) maintained on a high fat (HFD; 60%) diet. Glucose tolerance and area under the curve (inset) are shown. Oral glucose tolerance was assessed at 8 weeks in mice maintained on a normal chow diet ( E ). Intraperitoneal glucose (1 g/kg) tolerance and response to liraglutide (200 µg/kg) treatment ( F ) and insulin (0.75 units/kg) tolerance ( G ) was assessed at 20 weeks in mice maintained on a normal chow diet. ( H ) Fasting plasma glucagon concentration was measured on mice fed on a HFD (see Materials and Methods) n = 7–10 mice; * P ≤ 0.05.
Article Snippet: Animals were fed ad libitum with a
Techniques: Clinical Proteomics, Concentration Assay
Journal: Human Molecular Genetics
Article Title: Selective disruption of Tcf7l2 in the pancreatic β cell impairs secretory function and lowers β cell mass
doi: 10.1093/hmg/ddu553
Figure Lengend Snippet: Tcf7l2 deletion impairs glucose and GLP1-stimulated insulin secretion from isolated islets. ( A ) Plasma insulin following intraperitoneal injection of glucose from 20-week-old mice that had been maintained on a high fat diet was measured as described in Materials and Methods. ( B ) Real-time quantitative PCR analysis was performed on islets from 20-weeks-old mice that had been maintained on a normal chow diet. n = 7–10 mice; * P ≤ 0.05. ( C and D ) Insulin secretion as assessed in isolated islets from 20-week old Tcf7l2 fl/fl ::Ins1.Cre + (red) and littermate Tcf7l2 fl/fl ::Ins1.Cre − (black) mice on normal chow (C) or high fat diet (D). n = 5 mice per genotype; * P ≤ 0.05; ** P ≤ 0.01.
Article Snippet: Animals were fed ad libitum with a
Techniques: Isolation, Clinical Proteomics, Injection, Real-time Polymerase Chain Reaction
Journal: Human Molecular Genetics
Article Title: Selective disruption of Tcf7l2 in the pancreatic β cell impairs secretory function and lowers β cell mass
doi: 10.1093/hmg/ddu553
Figure Lengend Snippet: Impact of Tcf7l2 deletion on intra- and inter-cellular Ca 2+ dynamics. ( A and C ) Responses to glucose (17 m m ; G17) or GLP-1 (100 n m ) of β cells within intact islets of Tcf7l2 fl/fl ::Ins1.Cre + (red) and Tcf7l2 fl/fl ::Ins1.Cre − (black) mice maintained on a normal chow diet. ( B and D ) as for (A and C) but for islets from animals maintained for 12 weeks on a 60% fat diet. Correlation analysis ( , ) for islets from mice maintained on ( E ) normal diet and ( F ) high fat diet, and ( G ) quantitation of changes in typical islets from Tcf7l2 fl/fl ::Ins1.Cre + and Tcf7l2 fl/fl ::Ins1.Cre − mouse islets as indicated.
Article Snippet: Animals were fed ad libitum with a
Techniques: Quantitation Assay